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. 2008 Jan;172(1):225–235. doi: 10.2353/ajpath.2008.070065

Figure 3.

Figure 3

Stable expression of ARA70β and expression of cell cycle regulatory proteins in ARA70β-containing LNCaP cells. A: Western blots were performed with whole cell lysates prepared from LNCaP cells expressing ARA70β or LNCaP with vector control cells were cultured in the absence (−) or presence (+) of 10 nmol/L R881 (cell were treated for 24 hours). On adjusting the total protein concentration in each sample, the proteins were resolved on 10 or 12.5% SDS-PAGE, transferred to Immobilon paper, and the blots were probed with antibodies against AR, ARA70β, cyclin A, cyclin B, p27, and skp2. Each blot is representative of at least three independent experiments. B: Western blot showing increased expression of glucocorticoid receptor (GR), decreased E-cadherin, and PSA intracellular concentration in LNCaP cells overexpressing ARA70β (lane 5, LNCaP-pBabe; lane 6, LNCaP-ARA70β). C: Western blot showing expression of ARA70α (top) and β-actin (bottom) in PC3 (lane 7) and PC3-ARA70β cells (lane 8).