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. 2008 Jan;172(1):77–85. doi: 10.2353/ajpath.2008.070640

Figure 2.

Figure 2

Effect of low oxygen on Eng expression in first trimester villous explants. A: Expression of Eng mRNA in explants cultured at 3%, 8%, and 20% O2 measured by qRT-PCR (n = 6; *P < 0.05, 3% versus 20%). B: Top: Representative Western blot of Eng and sEng shows full-length Eng (90 kDa) and sEng (65 kDa), which are highly expressed in first trimester villous explants exposed to 3% O2 compared to 8% O2 and 20% O2; bottom: representative Western blot of HIF-1α. C: Eng protein densitometric analysis in first trimester villous explants exposed to different oxygen conditions, n = 6. *P < 0.05, 3% versus 20%.