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. 2008 Jan;172(1):98–111. doi: 10.2353/ajpath.2008.070404

Figure 7.

Figure 7

Expression of CCL2 by astrocytes in Krabbe-affected brain. A: Double-label confocal microscopy using CCL2 and GFAP showing scattered CCL2-expressing cells throughout the demyelinating area in the affected white matter (left). A minority of the CCL2+ cells are also GFAP+ and appear yellow. A higher magnification image is shown on the right, demonstrating colocalization (yellow) in one of several GFAP+ cells in the field. Images for individual channels are shown in the center of panel A CCL2 with Alexa 488 (green); GFAP conjugated with Cy3 (red); and DIC. B: Multiple CCL2-expressing cells also express the astrocyte marker peripherin and appear yellow (left). A higher magnification image is shown on the right, demonstrating clear colocalization (yellow) between CCL2 and peripherin in several cells. Images for individual channels are shown in the center of panel B. CCL2 with Alexa 488 (green); peripherin with alexa 488 (red); and DIC. C: CCL2 gene expression in the brain of affected rhesus macaques. A statistically significant (P < 0.0001) increase (∼221-fold) in CCL2 gene expression was observed in the brain of affected macaques compared with control macaques.