Table 2.
Tumor Spreading Produced by Local Tumor Growth and/or Metastasis Induced by Orthotopic Injection of Cells Transformed by Either RAC1wt or iRAC1 Compared with Control Cells SW620
| Cell line | Gene | No. of mice bearing tumor | Metastasis findings in tumor-bearing mice [n (%)]
|
|||
|---|---|---|---|---|---|---|
| Stage I | Stage II | Stage III | Stage IV | |||
| SW620 | None | 8 | 2 (25) | 0 (0) | 6 (75) | 0 (0) |
| wt1Clone | RAC1wt | 5 | 2 (40) | 1 (20) | 2 (40) | 0 (0) |
| wt2Clone | RAC1wt | 5 | 0 (0) | 0 (0) | 5 (100) | 0 (0) |
| i1Clone | iRAC1 | 1 | 1 (100) | 0 (0) | 0 (0) | 0 (0) |
| i2Clone | iRAC1 | 1 | 0 (0) | 0 (0) | 1 (100) | 0 (0) |
The tumor was staged in each animal as follows: stage I, local tumor site at the site of injection; stage II, stage I plus foci of tumor in the visceral and parietal peritoneum; stage III, stage I or II plus mesenteric lymph node and/or pancreatic metastasis; stage IV, stage I, II, or III plus hepatic and/or lung metastasis.