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Involvement of Frizzled 4 and 7 in sFRP-1-induced endothelial cell spreading. Forty-eight hours after transfection with siRNA control or indicated Frizzled siRNA (Fzd2, Fzd4, Fzd5, Fzd6, Fzd7), MS1 cells were submitted to a spreading assay on wells coated with type I collagen. a: The percentage of spread cells were scored as described in the Materials and Methods section. b: F-actin (red fluorescence) distribution in adherent MS1 cells was detected by rhodamine-phalloidin.
