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. 2008 Jan;172(1):37–49. doi: 10.2353/ajpath.2008.070130

Figure 6.

Figure 6

sFRP-1 induction of EC spreading is independent of β-catenin and requires GSK-3β activation. Endothelial cell lines expressing β-catenin (+/+) or deleted for β-catenin (−/−) as demonstrated by Western blot, were treated (▪) or not (□) with rb sFRP-1 for 30 minutes in serum-free medium. The role of GSK-3β was investigated in ECs deleted for β-catenin by infection of ECs with adenovirus expressing GSK-3β-KM. After stimulation, spreading assays on type I collagen were performed as described in the Materials and Methods section. Data are mean percent spreading ± SD. *P < 0.05 relative to control without recombinant bovine sFRP-1.