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Activity of cGK-1 as reflected by levels of phosphorylated VAsodilator Stimulated Phosphoprotein at serine 239 (P-VASP) in response to acute challenges with organic nitrates. Aortic segments of C57B6 (WT) and ALDH-2−/− (ALDH-2 ko) were incubated with PEDN, PETN or solvent (DMSO). Bars reflect densitometric analysis. The Western blot is representative of five independent experiments. $P<0.05 vs WT DMSO; #P<0.05 vs WT DMSO, ALDH-2 ko DMSO and ALDH-2 ko PETN, NS vs WT PETN and WT PEDN.
