Figure 4.

(a) Chemiluminescence nitrogen detection (CLND) of PETN and PEDN in suspensions of isolated mitochondria. Bars reflect the amount of recovered organic nitrate by CLND analysis after incubation of isolated mouse liver mitochondria with the concentration shown on the x-axis. ^*P<0.05 vs ALDH-2 ko; n.d.: not detected, e.g. peaks were below the level of quantification and therefore not elegible for analysis. (b) Activity of ALDH-2 was assessed by measuring the conversion of 2-hydroxy-3-nitro-benzaldehyde to the benzoic acid product (2H3N-BA) in isolated mitochondria. Insert shows a Western blot of ALDH-2 in extracts of aortic tissue. ^*P<0.05 vs WT.