Table 1.
Potency and efficacy of vasodilator-induced relaxation in isolated aortic rings from WT- vs ALDH-2−/− mice
| PETN | PETN+10 μM Benomyl | PETriN | PEDN | PEDN+10 μM Benomyl | PEMN | PETN+3 μM ODQ | GTN | 1,3-GDN | ACh | |
|---|---|---|---|---|---|---|---|---|---|---|
| WTi | 6.77±0.09 (n=7) | 6.12±0.23a,b (n=8) | 5.51±0.20 (n=10) | 3.89±0.07a (n=11) | 3.98±0.18c (n=7) | 3.24±0.03 (n=12) | 4.83±0.03a (n=8) | 7.24±0.08 (n=8) | 4.26±0.11 (n=8) | 7.19±0.07 (n=17) |
| ALDH-2−/−i | 5.84±0.09a (n=12) | 5.77±0.27a,b (n=6) | 4.69±0.08d (n=12) | 3.84±0.08c (n=12) | 4.25±0.17c (n=6) | 3.17±0.03e (n=11) | 4.86±0.03a (n=8) | 5.87±0.08f (n=7) | 3.92±0.16 g (n=8) | 7.22±0.05 h (n=19) |
| WTj | 85.6±1.14 (n=7) | 77.2±2.32a,b (n=8) | 95.8±1.21 (n=10) | 85.2±2.19a (n=11) | 76.8±0.18c (n=7) | 68.7±6.76 (n=12) | 10.9±5.72a (n=12) | 89.8±1.85 (n=8) | 91.03±1.64 (n=8) | 78.0±3.01 (n=17) |
| ALDH-2−/−j | 77.5±1.10a (n=12) | 75.3±3.21a,b (n=6) | 83.2±2.80d (n=12) | 83.9±1.29c (n=12) | 78.2±8.51c (n=6) | 65.9±2.66e (n=11) | 15.8±5.61a (n=12) | 77.9±3.49f (n=7) | 93.44±2.10 g (n=8) | 76.8±2.43 h (n=19) |
Abbreviations: ALDH, aldehyde dehydrogenase; 1,3-GDN, 1,3-glyceryl dinitrate; NS, not significant; PEDN, pentaerithrityl dinitrate; WT, wild type.
a
P<0.05 vs WT PETN.
b
n.s. vs ALDH-2−/− PETN.
c
n.s. vs WT PEDN.
d
P<0.05 vs WT PETriN.
e
n.s. vs WT PEMN.
f
P<0.05 vs WT GTN.
g
n.s. vs WT 1,3-GDN.
h
n.s. vs WT Ach; (n) indicates number of experiments.
i
The values shown are –log(EC50) [M] of concentration–relaxation curves±s.e.m.
j
Maximal relaxation (% relaxation) of concentration–relaxation-curves±s.e.m.