Figure 3.

Depletion of descending NA and 5-HT. To deplete NA or 5-HT, 6-OHDA or PCPA was injected intracisternally once or administered i.p. for 5 consecutive days, respectively. After assessment of thermal and mechanical nociception, the contents of NA, 5-HT and DA in the brainstem (B) and spinal cord (S) obtained from vehicle control (n=6 each) and 6-OHDA (a)- or PCPA (b)-treated mice (n=18 each) were measured using reverse-phase high-performance liquid chromatography with electrochemical detection.