Figure 8.

Neither blockade of spinal α₂-adrenergic nor 5-HT_1A receptors affects the increased locomotor activity elicited by i.c.v. administered taltirelin. Mice were injected i.c.v. with taltirelin (tal, 0.3 μg) 15 min after i.t. injection of either yohimbine HCl (yoh, 3 μg) or WAY-100635 (WAY, 10 μg), and the assessment of locomotor activities was carried out for 1 h post injection (n=9 each). Ordinates: locomotion measured in 5-min periods (a) and between 0–30 and 30–60 min after injection (b). Taltirelin was administered at time zero. In (b), the significance of differences between the saline i.t.+taltirelin i.c.v. group and other groups (saline i.t.+saline i.c.v., yoh i.t.+tal i.c.v., WAY i.t.+tal i.c.v.) was determined by two-tailed non-parametric Bonferroni-type multiple comparisons following the Kruskal–Wallis test (three comparisons in four groups, ^*P<0.05). ns; not significant.