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Polymyxin B but not PMBN modulates P2X7 oligomerization. Western blot analysis was carried out as described in Methods. Cells were preincubated for 15 min in the presence of polymyxin B or PMBN, then nucleotides were added and incubation carried out over further 30 min. ATP concentration was 1 mM, while BzATP was 100 μm; wtHEK293 cells were used as a negative control for P2X7 receptor expression. A representative Western blot of three similar is shown.
