Table 1.

Primer sequences and assay conditions of RT-PCR

Genes	Sense primer (5′–3′)	Antisense primer (5′–3′)	Annealing temperature (°C)	Cycles
S1P1	AAGACCTGTGACATCCTCTTC	ATGAACCCTTTAGGAGCTTGACAA	55	22
S1P3	GACTGCTCTACCATCTTGCCC	ATGAACACGCTCACCACAAT	55	32
SR-BI	CACTACGCGCAGTATGTGCT	TGGCACTGGTGGGCTGTC	57	28
GAPDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA	55	21

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; S1P, Sphingosine 1-phosphate.