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Cre-mediated recombination in vivo. A Southern blot of HindIII and PstI-digested yeast DNA probed with a GAL4 AD fragment is shown. Lane 1: Bait and prey vectors in yeast strain AH109 in the absence of Cre expression vector (AH109-pCDlox66, pGADt7lox71). Lane 2: Bait and prey vectors in yeast strain AH109 in the presence of Cre expression vector (AH109-pCDlox66, pGADt7lox71, pFA6a2 μ-Adc1Cre).
