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BI-Tag method diagram. (A) Bait and prey BI-Tag Y2H vectors with lox66 and lox71, respectively. (B) Cre induced recombination at lox sites causes linkage of the BI-Tag Y2H vectors. (C) MmeI digestion generates the BI-Tag-containing lox66/71 flanked by MmeI and 20 bp sequence tags. (D) NotI linkers are ligated to the BI-Tag, and PCR is performed. (E) After NotI digestion the BI-Tags are ligated to form concatamers.
