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. 2007 Nov 5;35(21):e141. doi: 10.1093/nar/gkm894

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — BI-Tag method. (A) PCR amplification (with primers that anneal to GAL4 AD and DBD cDNAs) across linked cDNAs and lox sequence of the HoxA1 Y2H positive colony DNA (bar). (B) MmeI digestion of the PCR product to produce the 86 bp BI-Tag (arrow). (C) Left lane: 160 bp PCR product that includes the BI-Tag and 40 bp linkers (arrow), Middle lane: 94 bp BI-Tag (arrow) generated by NotI digestion with NotI compatible overhangs for concatenation. (D) Amplicons of BI-Tag concatamer inserts in a cloning vector.