Table 2.
The mean Emax values±s.e.m. and the mean EC50 values, with 95% CI in parentheses for the efficacy of tested cannabinoids in mouse brain or hCB2-CHO cell membranes
| Cannabinoid | Membrane preparation | Emax % stimulation above basal | EC50 (95% confidence intervals) | n |
|---|---|---|---|---|
| CP55940 | Mouse brain | 85±3 | 11 nM (7 and 17 nM) | 9 |
| R-(+)-WIN55212 | 94±3 | 140 nM (94 and 209 nM) | 7–8 | |
| Rimonabant | −17±3 | 351 nM (32 and 3901 nM) | 7–8 | |
| Cannabidiol | ND | ND | ||
| O-2654 | ND | ND | ||
| CP55940 | HCB2-CHO cell | 43±3 | 0.5 nM (0.2 and 1.9 nM) | 6–12 |
| SR144528 | −16±1 | 0.8 nM (0.2 and 2.4 nM) | 5 | |
| Cannabidiol | −15±5 | 503 nM (22 nM and 11 μm) | 8 | |
| O-2654 | −17±3 | 240 nM (37 and 1570 nM) | 6 | |
Abbreviations: CI, confidence intervals; hCB2-CHO, Chinese hamster ovary cells stably transfected with human CB2 receptors; ND, not defined; R-(+)-WIN55212, (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo-[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone; SR144528, N-[(1S)-endo-1,3,3-trimethyl bicyclo [2.2.1]heptan-2-yl]-5-(4-chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide.