Figure 2.

COX activity in SH-SY5Y neuroblastoma cell line. (a) Left panel, COX activity is expressed as PGE₂ generated by the microsomal fraction obtained from SH-SY5Y cells treated with vehicle (v) or staurosporine (st; 500 nM; 24 h). (a) Right panel, the PGE₂ levels released into supernatants obtained from vehicle-(v) or staurosporine (St; 500 nM)-treated SH-SY5Y cells for 24 h. Data represent mean+s.e.m. of 12 experiments. ^***P<0.001 as compared with vehicle. (b) Effect of indomethacin, NS-398 and acetaminophen on COX activity measured in microsomal fraction obtained from vehicle-treated SH-SY5Y cells. Data represent mean+s.e.m. of 12 experiments. ^***P<0.001 ^**P<0.01, compared to vehicle-treated cells. (c) Effect of indomethacin, NS-398 and acetaminophen on COX activity measured in microsomal fraction obtained from staurosporine (500 nM)-treated SH-SY5Y cells for 24 h. Data represent mean±s.e.m. of 12 experiments. ^***P<0.001; ^*P<0.05 in comparison to staurosporine-treated cells.