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. 2000 Oct 2;151(1):95–106. doi: 10.1083/jcb.151.1.95

Figure 2.

Figure 2

FISH with a pan-centromere probe (fluorescein, green) and a pan-telomere probe (rhodamine, red) to undisrupted diploid nuclei (DAPI, blue) of wild-type and ndj1Δ SK1 cells. (a) Vegetative wild-type nucleus before induction of meiosis (t = 0 min) displays clustered centromeres that form a single green signal, while telomeres form four perinuclear clusters. (b) Premeiotic ndj1Δ nucleus with one centromere cluster and three large telomere clusters at the opposite pole of the nucleus, thereby resembling a Rabl orientation. (c) WT and (d) ndj1Δ meiocyte nuclei from two later time points (200 and 260 min, respectively) displaying centromere and telomere signals distributed throughout the nuclei. Few centromere signals are dissociated from the centromere cluster in nucleus (c), while in nucleus (d) exhibits dispersed centromere signals. Bar, 5 μm.