Figure 6.
Effects of vanadate and AMP-PNP on vesicle motility and fission. MTs were bound to the glass chamber via DEAE-dextran. Studies were performed at ATP concentrations of 50 μM (black bars) or 4 mM (cross-hatched bars) in the absence of a regenerating system. The bars in the left graph indicate the percentage of MT-bound vesicles that moved upon ATP addition in the presence or absence of varied concentrations of vanadate or 1 mM AMP-PNP. The total number of MT-bound vesicles that were examined in each experiment is in parentheses. The bars in the right graph indicate the percentage of moving vesicles that underwent fission.