Table 1.
Event number | Ligand vesicle 1 | Ligand vesicle 2 |
---|---|---|
1 | 94.2 | 5.8 |
2 | 81.1 | 18.9 |
3 | 79.0 | 21.0 |
4 | 78.7 | 21.3 |
5 | 74.7 | 25.3 |
6 | 67.9 | 32.1 |
7 | 67.9 | 32.1 |
8 | 66.8 | 33.2 |
9 | 66.2 | 33.8 |
10 | 65.2 | 34.8 |
11 | 64.2 | 35.8 |
12 | 62.5 | 37.5 |
13 | 59.4 | 40.6 |
14 | 58.9 | 41.1 |
15 | 54.5 | 45.5 |
16 | 51.8 | 48.2 |
17 | 50.7 | 49.3 |
Mean ± SEM | 67.3 ± 2.9 | 32.7 ± 2.9 |
Results are expressed as a percentage of the total recovered fluorescence for 17 randomly selected endocytic vesicles that underwent fission. In each row, the vesicle with the majority of ligand fluorescence was designated vesicle 1. In these experiments, receptor was not monitored.