Figure 3.

Effects of ciglitazone upon the uptake of [³H]AEA. The plates containing either C6 cells at pH 7.4 (a) C6 cells at pH 6.2 (b) or RBL2H3 cells at pH 7.4 (c) or wells alone (all three panels) were preincubated with either vehicle or URB597 for 10 min followed by addition of ciglitazone and 10 min incubation. [Ara-³H]AEA was added and uptake was allowed for 5 min. URB597 concentrations were 0.1 μM for (a and b), and 1 μM for (c). [Ara-³H]AEA concentrations were 0.1 μM for (a and b), and 0.2 μM for (c). In each case, the total size of the column represents the uptake. Values shown are means±s.e.m., n=6. ^*P<0.05 vs the respective control value, Dunnett's multiple comparison test following significant one-way analysis of variance (ANOVA) for repeated measures for the compound. AEA, anandamide (arachidonoylethanolamide); URB597, 3′-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate.