Table 2.

Cardiac NOS activities

	Nondiabetic untreated	Diabetic untreated	Diabetic atrasentan-treated	Diabetic ramipril-treated	Diabetic combination-treated
Total activity	1.22±0.090	1.03±0.092	1.08±0.095	1.20±0.031	1.20±0.027
1400 W (10 μM)	1.23±0.093	1.07±0.094	1.04±0.063	1.22±0.043	1.18±0.023
EDTA (5 mM)	−0.02±0.020	−0.01±0.026	−0.03±0.014	0.03±0.013	−0.02±0.042

Abbreviation: EDTA, ethylenediaminetetraacetic acid.

Enzyme activity is given as pmol [³H]L-citrulline formed min⁻¹ (mg protein)⁻¹. Data are mean±s.e.m. (n=5 per group). Activities obtained in the presence of 1400 W were not significantly different from total activity (P=NS).