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. 2007 Jun 25;151(8):1176–1186. doi: 10.1038/sj.bjp.0707335

Figure 2.

Figure 2

Cisplatin (CDDP)-induced [Ca2+]i rise depends on extracellular calcium and IP3 receptors but not calcium release from stores. (a) Left side: in HeLa-S3 cells, [Ca2+]i did not increase when no calcium was added to the extracellular solution. Right side: averaged increase in [Ca2+]i by cisplatin (10 μM) in HeLa-S3 is highly significantly (***P<0.001; means±s.d., n=27) reduced when no calcium is added to the extracellular solution. (b) Left side: averaged time course of the [Ca2+]i concentration in HeLa-S3 during the application of cisplatin (1 μM) and with the coapplication of the IP3-receptor antagonist 2-APB (50 μM). Right side: with the coapplication of 2-APB, the [Ca2+]i elevation is highly significantly reduced (***P<0.001; means±s.d., n=41). (c) Left side: pre-application of caffeine (10 mM) did not abolish the CDDP-induced [Ca2+]i elevation (time course of averaged data; left side). Right side: summary of caffeine-induced rise in [Ca2+]i and the further CDDP induced increase (*P<0.05; ***P<0.001; means±s.d., n=19). [Ca2+]i, intracellular calcium; IP3 receptors, inositol-1,4,5-trisphosphate receptors; 2-APB, 2-aminoethoxydiphenyl borate.