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. 2007 Jun 18;151(8):1167–1175. doi: 10.1038/sj.bjp.0707342

Figure 2.

Figure 2

Western blot analysis of Trx1 and TrxR1 levels. (a) HT29 cells and (b) MRCV cells were treated with varying concentrations of PMX464 for 72 h (final 48 h either under normoxic or hypoxic [1% O2] conditions; hypoxic data not shown for HT29). Blots were probed simultaneously for TrxR1 (∼58 kDa) and Trx1 (∼12 kDa), and stripped and reprobed for β-actin (∼42 kDa) as a loading control. PMX464, 4-(benzothiazol-2-yl)-4-hydroxycyclohexa-2,5-dienone; Trx1, thioredoxin; TrxR1, thioredoxin reductase.