Figure 2.

FACS analysis of follicle cells. Cells are plotted as fluorescence events on the axes shown. (A) Wild-type follicle cells. There are very few events in the region of high GFP fluorescence. Arrows indicate concentrated regions along the Hoechst axis roughly corresponding to the four major ploidies in the population. (B) A62 GAL4/UAS-GFP. A population of GFP-positive cells can clearly be distinguished. The rectangle represents the approximate sort window used to select GFP-positive cells. (C) Live/dead selection. The approximate sort window used to select live cells is overlaid on the plot. The upper streak, which is high in propidium iodide fluorescence, represents dead cells, which are excluded from the sorted population. The separation of cells of different ploidies can most easily be seen in the dead cells (arrows), whose exposed nuclei can readily equilibrate with the Hoechst dye.