Table 1.
cDNA comparison method | Cell types compared | No. of probes selected for secondary screening | No. of probes showing anticipated follicle cell patterns | Differentially expressed genes of interest | Enriched in | Novel follicle cell gene? | Fig. 4 ref. |
---|---|---|---|---|---|---|---|
Microarray | A62+ vs. A62− | 11 | 4* | GM07659 (FKBP-12 homologue) | A62− | Yes | A–C |
GM04985 (novel E75 transcript) | A62+ | Yes | D | ||||
Differential display | A62+ vs. 55B+ | 23 | 1 | VM34C | 55B+ | No | E and F |
grk/grk vs. grk/+ | 16 | 1 | E78B | grk/+ | Yes | G–J | |
grk/grk vs. grk/+ (no FACS) | 11 | 1 | DSbf1 | grk/grk | Yes | K–N |
Includes two clones of lesser interest because they show temporal but not spatial regulation, reflecting the stage specificity of A62 GAL4.