Figure 2.

C-18 inhibits Ang-1-induced human umbilical vein endothelial cell (HUVEC) migration. (a) Cells were placed in transwells and allowed to migrate for 4 h in the presence of C-18 (1 or 100 pM), Ang-1 (250 ng ml⁻¹) or both. Migrated HUVEC were fixed, stained and counted in eight random fields. Data are expressed as means±s.e.mean; n=5; ^*P<0.05 vs vehicle. Representative photomicrographs of migrated cells at × 100 magnification (bottom). (b) HUVECs were serum-starved, and Ang-1 (250 ng ml⁻¹) was added as a survival factor in the presence or absence of C-18 (100 pM). After 24 h, caspase-3 activity was determined as an index of apoptosis according to the manufacturers' instructions. Data are expressed as means±s.e.mean; n=4; ^*P<0.05 vs vehicle. Ang, angiopoietin; C-18, 2-((1E,Z)-1-benzylidene-5-bromo-2-methyl-1H-inden-3-yl)acetic acid.