Figure 4.

C-18 inhibits human umbilical vein endothelial cell (HUVEC) migration in response to a variety of growth factors. Cells were placed in transwells and allowed to migrate for 4 h in the presence of fibroblast growth factor (FGF)-2 (10 ng ml⁻¹), vascular endothelial growth factor (VEGF; 50 ng ml⁻¹) or BAY 41-2272 (0.1 μM) with or without C-18 (100 pM). Migrated HUVECs were fixed, stained and counted in eight random fields. Data are expressed as means±s.e.mean; n=5; ^*P<0.05 vs vehicle; ^#P<0.05 vs control. C-18, 2-((1E,Z)-1-benzylidene-5-bromo-2-methyl-1H-inden-3-yl)acetic acid.