Figure 4.

Responses to ECLs in wild-type C57bl mice in vivo. (a) Lipolysis, as assessed by plasma-free fatty acid (FFA) levels, was determined 60 min following a single 10 mg kg⁻¹ i.p. injection of the ECL. Data are means±s.e.mean from four (vehicle and noladin) or five (OEA and WIN 55212-2) determinations. ^*P<0.05, ^**P<0.01 vs vehicle treatment, one-way analysis of variance with Dunnett's multiple comparison test. (b) Influence of repeated injection of OEA (3 days at 10 mg kg⁻¹ per day i.p.) administration on levels of inflammatory modulator expression in the mouse cerebral cortex. Data are means±s.e.mean of three determinations of immunoreactivity expressed as a percentage of actin immunoreactivity from the same samples. ^*P<0.05, ^**P<0.01 vs vehicle treatment, by Student's t-test. ECL, endogenous cannabinoid-like compound; OEA, N-oleoylethanolamine.