Fig. 3.

TEM evaluation of 18-hour CIT-OLTx livers. Control LSECs display typical fenestrated shape and close association with the hepatocyte microvilli and the space of Disse. After 18-hour CIT, many LSECs are necrotic (arrowheads) and microvilli within the space of Disse are greatly reduced; however, hepatocytes appear to be normal. At 1–6 hours post-OLTx, the entire sinusoid surface is essentially devoid of intact, recognizable LSECs (arrowheads). Hepatocyte microvilli are essentially absent and platelet deposition is apparent (P), as are macrophages (M) with cellular debris inclusions (arrow) and leukocyte adherence (L). Collagen bundles (C) within the space of Disse remain in many areas. By 24 hours post-OLTx, LSECs have largely recovered, as has the ultrastructure within the space of Disse. Macrophages at this time show large numbers of cellular inclusions packed with cellular debris (arrows). Bars = 2 μm. Data are representitive of 2 separate experiments.