Fig. 6.

EGFP⁺ BM-derived recipient cells integrating into WT donor livers. (A) Liver harvested from a WT liver transplanted into an EGFP⁺ recipient 36 days after 18-hour CIT and OLTx. Liver was stained for nucleus (blue) and SE-1 (red). Many EGFP⁺ cells are scattered throughout the liver, including an EGFP⁺ hepatocyte (arrow) as well as many perivascular cells surrounding the portal vein (PV, small arrows) and intrasinusoidal cells. (B) The box from panel A is magnified to show the EGFP⁺ hepatocyte (arrow) as well as an EGFP⁺ LSEC (box). (C) The box from panel B is magnified to show SE-1⁺ plasma membrane staining (red) and the EGFP⁺ and flattened nucleus (blue) of a BM-derived LSEC. (D) Same area as panel C, with the EGFP signal removed to show contiguous SE-1 plasma membrane labeling surrounding a flattened nucleus (blue). (E) Midlobular area colocalizing CD45⁺ (red) and EGFP⁺ cells (arrows). Most EGFP⁺ cells are leukocytes. A few CD45⁺ cells are not EGFP⁺ (arrowheads). (F) Midlobular area colocalizing ED1⁺ cells (CD68, macrophages, Kupffer cells; red) with EGFP⁺ cells. Very few ED1⁺ cells are also EGFP⁺ (arrows), but most are EGFP⁺ (arrowheads). These data correlate well with the FACS data from isolated sinusoidal cells presented in Table 3.