Table 2.

Flow Cytometric Analysis of Cationic Colloidal Magnetite-Isolated Sinusoidal Cells Obtained from WT 18-Hour CIT Liver Transplanted into EGFP Recipients

Cell Type	Cell Marker	Percentage of Total Cells Isolated from Sinusoid*	Percentage of EGFP+ Cells†
LSECs	SE-1	42.0 ± 5.0	4.1 ± 2.1
Large vessel endothelial cells/LSECs	CD31	43.8 ± 7.0	0.4 ± 0.3
Leukocytes	CD45	54.6 ± 7.5	41.2 ± 2.8
Macrophages	ED2 (CD163)	9.30 ± 8.5	4.1 ± 3.6

Sinusoidal cells were isolated 3–7 days after WT liver (18-hour CIT) OLTx into EGFP⁺ recipients using the cationic magnetite isolation protocol. Cells were then analyzed for specific cell markers* using FACS and for dual positive cell marker/EGFP signal.

^†Results are given as the mean ± standard deviation (n = 3).