Table 3.

Characterization of Cells Recovered from CIT Liver Exudates

CIT	n	Total Cells (× 105/g Liver)	Total Cells (× 106/Liver)	SE-1+ (%)	CD45+ (%)	SE-1+/Annexin V+ (%)
0 hours	2	0.4–3.3	0.96–2.30	41.0–45.5	54.4–58.9	5.7–9.4
18 hours	3	0.3–3.8	0.56–3.96	30.2–54.9	45.2–69.8	4.1–13.1
48 hours	2	0.5–1.9	0.57–14.27	51.4–68.8	31.2–48.6	13.2–14.5

Cells were collected from liver University of Wisconsin storage solution following 0, 18, and 48 hours of CIT and analyzed using FACS for SE-1, CD45, and SE-1⁺/annexin V⁺ signals. Both LSECs (SE-1⁺) and leukocytes (CD45⁺) were washed out of the liver after CIT. A small percentage of LSECs were apoptotic as indicated by annexin V staining. Liver graft weights from rats were routinely between 9 and 11 g.