Panel A displays the hybridization cartridge assembled by Affymetrix, Inc., which contains a solid support featuring 25-mer probes designed to sequence 26,725 nucleotides (bases) of SERPINA1, JAG1, ATP8B1, ABCB11, and ABCB4. Panel B shows signals obtained from a high-resolution scanning after hybridization of a chip with amplicons from a subject. In the top portion of panel C, magnification of a region of the chip shows that the brightest signal for each set of four 25-mer probes for each column identifies a base. In the lower panel, identical base calls were made by standard capillary sequencing methodology.