Table II.

RT-PCR primers, cycle no., and hybridization temperatures

Gene	Primer Set	No. of Cycles	Hybridization Temperature
ACS2 (At1g01480)	5′AGATCGTCGAGAAAGCATCTG3′	30	56°C
	5′GAAGAGGTGAGTGTGGTGAC3′
ACS4	5′GTTTACGAAGTGAAGCTCAAC3′	35	56°C
	5′GTCTCATCAATCATGTTCGCG3′
ACS5	5′GCGGCAAGTCTCAAGAGGA3′	28	54°C
	5′TTCTGGGCTTGTTGGTAAGC3′
ACS6	5′CTGAATCTATTGTCTAAAATCGC3′	30	55°C
	5′ACGCATCAAATCTCCACAAAG3′
ACS7	5′TATTTTGTTGGATGAATTTGGGT3′	30	56°C
	5′TTCTCTTCAACGCAATCTCC3′
ACS8	5′GTCCAGTTTCGGTCTAATCTC3′	28	55°C
	5′ATAGGTGTCTCATGTCAACCC3′
ACS9	5′TCGGTTTACCAGGTTTTCGC3′	30	55°C
	5′ACACGAGTTTCTTCTGACGAA3′
ACS10	5′ACAGGCAGAGATTGCAGAG3′	30	55°C
	5′ACTGAAACAGATACGGAACC3′
ACS11	5′CAGTTGTTTGAAGAGTAACGC3′	30	55°C
	5′TAACAGGAAAGCTTGGAGA3′
ACS12	5′AGAGCTGGAGTCATCTACTCC3′	30	55°C
	5′GCAAGCTGTCTGATTCGTTCC3′
ACO (At5g63600)	5′CCTGTCTACTGAAAACCCTC3′	30	56°C
	5′GTCTCCTTGAACAATTCATCA3′
ACO (At1g04350)	5′GCATTCACTAAAATTATACA3′	28	56°C
	5′CAAATAAGTAAACCATTTCCT3′
ACO (At1g05010)	5′GATCTGCTGTGCGAGAATCTC3′	28	56°C
	5′TAAATAACCCTTCTCTAAACC3′
ACO (At1g62380)	5′CCAGCTACTTCGCTTGTCGAG3′	27	56°C
	5′GTCTCTACGGCTGCTGTAGGA3′
ACO (At2g19590)	5′CAGAGGAACTCAGCAAGACG3′	28	56°C
	5′ATCCGTATGTTCTCTCAGCC3′
ACO (At5g43450)	5′GTACAAAGATATCACCATACCAG3′	28	56°C
	5′TGGTTGAGGAACTCTATAGC3′
UBQ14	5′GATCCAGGACAAGGAAGGTATC3′	25	55°C
	5′AGCCTTAGCACCAAGTGAAGG3′