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. 2003 Oct;133(2):560–570. doi: 10.1104/pp.103.024810

Figure 1.

Figure 1.

Molecular cloning of the rice BBI gene family. A, Diagram of rice chromosome 1. The RBBI gene cluster is located on the southern end of this chromosome as indicated by the arrow near the genetic marker, RG612. B, Southern blot using RBBI8 (see “Materials and Methods”) insert (the conserved coding region of RBBI2-3, 450 bp in length) as a probe. Rice genomic DNA was digested with BamHI (1), XhoI (2), BamHI + XhoI (3), and BamHI + EcoRV (4). C, Diagram showing the structural composition of the RBBI family. D, Composition of the RBBI gene cluster on chromosome 1. The arrowhead indicates the directions of the ORFs. Bar = 1 kb. E, Alignment of the conserved Cys-rich domains. Gaps are indicated by a dash. Putative trypsin- and/or chymotrypsin-reactive sites are indicated in blue letters, and the conserved Cys residues are in red.