Figure 3.
Northern-blot analysis of spatial and temporal pattern of GhWBC1 expression. A, Expression in seedlings (10 d old) and developing ovules (9 DPA); total RNA was isolated from roots (1), hypocotyls (2), cotyledons (3), young true leaves (4), fiber cells (5), wild-type intact ovules (6), and fl ovules (7); 20 μg of total RNA was loaded each lane. B, Expression of GhWBC1 in developing fibers of 5, 9, 15, 20, and 25 DPA, respectively; 1.25 μg of total RNA was loaded each lane. C and D, expression of GhWBC1, E6, and Expansin genes in li mutant and wild-type cotton cv Xu-142 ovules. C, GhWBC1 expression in 1- and 2-DPA ovules; D, GhWBC1, E6, and Expansin expression in 5- and 9-DPA ovules. Ten micrograms of total RNA was loaded each lane.