Figure 3.

Enhanced resistance of ZntA-expressing Arabidopsis plants to Pb(II) or Cd(II). A, RNA gel-blot analysis of whole seedlings of four ZntA-transgenic T₃ homozygous lines. Hybridization with an EF1-α probe was used as a loading control. B and C, Photographs of wild-type and ZntA-transgenic T₃ homozygous plants. Seeds were germinated and grown on one-half Murashige and Skoog-agar medium with 3% (w/v) Suc for 2 weeks and then transferred onto small gravels wetted with one-half Murashige and Skoog liquid media plus 3% (w/v) Suc containing 0.7 mm Pb-tartarate (B) or 70 μm CdCl₂ (C).