Table I.

Primers used for the isolation of the promoter region and the 3′ end of the copper chaperone for a Cu/Zn Sodp (StCCS) gene and for the determination of the TIS

The first part of the table indicates the primers used in the three genome walks to isolate the promoter region of StCCS. AP1 stands for primary and AP2 for nested adaptor-related primer, GSP with odd numbers for primary gene-specific primer, and GSP with even numbers for nested gene-specific primer. In the second part of the table, the primers used in the GeneRacer kit to isolate the StCCS TIS and the 3′ region are depicted. GR3′/5′ stands for primary adaptor-related primers, GR3′/5′N for nested adaptor-related primers, GSP with odd numbers for primary gene-specific primer, and GSP with even numbers for nested gene-specific primer.

Experiment	Primer Name	Primer Sequence (5′→3′)
Walk No.
1, 2, and 3	AP1	GTAATACGACTCACTATAGGGC
1, 2, and 3	AP2	ACTATAGGGCACGCGTGGT
1	GSP1	AATATCTGGTCCTTTGAATTCGGCAAC
1	GSP2	GCAGCAGATATAAGGAAATCTTCGGGT
2	GSP3	CCCCCAAACTAAACCTTCAATAACCAA
2	GSP4	AAATGGGCTACATTAAGCCATCAAGAT
3	GSP5	CGGAGGAAGTTTGGTGATTGGAAGAAG
3	GSP6	CGATGGACGTTCAAACTGCGAAGAAGA
Isolation of 3″ or TIS
3′ and TIS	GR5′	CGACTGGAGCACGAGGACACTGA
3′ and TIS	GR5′N	GGACACTGACATGGACTGAAGGAGTA
3′ and TIS	GR3	GCTGTCAACGATACGCTACGTAACG
3′ and TIS	GR3′N	CGCTACGTAACGGCATGACAGTG
TIS	GSP7	GAAGGCGTTTCCATATGAAGAGCTGAA
TIS	GSP8	GAAGCAGCAAAAGCAGCAGCTGGAATA
3′	GSP9	AGCCCGTTTGATTGGACAAGGAGTACC
3′	GSP10	TATCTGCTGCTGTTGCCGAATTCAAAG