Table VI.
Relative expression level of M. truncatula transcripts based on the repetitive occurrence of sequences in the EST data sets from immature seeds
The cluster accession nos. of the M. truncatula ESTs identified by MS were extracted from the Medicago EST Navigation System (http://medicago.toulouse.inra.fr/) release of January 2003. The cluster accession nos. were used to search the no. of sequences from the same mRNA in two different data sets: a cDNA library of immature seeds ranging in age from 11 to 19 DAP (MtGESD, 4,525 ESTs) and a cDNA library of immature seeds ranging in age from 25 to 35 DAP (MtGLSD, 4,866 ESTs). The M. truncatula transcripts whose expression was found to be significantly variable in the two different data sets (χ2 test, P < 0.050) were grouped according to their expression profiles during seed development. In each class of proteins (see Table I), the corresponding M. truncatula transcripts were sorted in the descending order based on their EST counts in both data sets.
| Protein Class | Strongest BLAST Hit | EST gi | Cluster Accession No. | MtGESD (11-19 DAP) | MtGLSD (25-35 DAP) |
|---|---|---|---|---|---|
| Transcripts preferentially expressed during early stages of seed development | |||||
| 1 | Vicilin | 14986367 | MtC60032.3_GC | 282 | 55 |
| 1 | Vicilin | 14984555 | MtC60159_GC | 41 | 21 |
| 1 | Chlorophyll a/b binding | 13783768 | MtC00119_GC | 10 | 0 |
| 2 | Vicilin | 14984749 | MtC60032.4_GC | 280 | 55 |
| 2 | Annexin | 7718287 | MtC20316_GC | 14 | 1 |
| 2 | S-adenosylmethionine synthetase | 11609660 | MtC00046_GC | 4 | 0 |
| 3 | Flavonone 3-hydroxylase | 13596069 | MtC93321_GC | 12 | 0 |
| 3 | Protein disulfide isomerase | 10697964 | MtC10403_GC | 10 | 0 |
| 3 | GAPDHc | 9671653 | MtC00021.1_GC | 4 | 0 |
| Transcripts preferentially expressed during late stages of seed development | |||||
| 1 | Legumin | 14985010 | MtC60042_GC | 119 | 451 |
| 1 | Legumin | 14986270 | MtC60076_GC | 67 | 311 |
| 1 | Convicilin | 14985541 | MtC60090_GC | 71 | 209 |
| Transcripts whose relative levels did not vary significantly during seed development | |||||
| 1 | Lipoxygenase | 14982738 | MtC60669_GC | 11 | 15 |
| 1 | ACC oxidase | 10520454 | MtC10108.2_GC | 9 | 6 |
| 1 | Met synthase | 9669628 | MtC00018_GC | 4 | 6 |
| 1 | Homology not assigned | 13595945 | MtD00176_GC | 1 | 2 |
| 1 | Glyoxalase 1 | 11897629 | MtC60084_GC | 3 | 0 |
| 1 | Oxygen-evolving enhancer | 11902222 | MtC60015_GC | 2 | 1 |
| 1 | Starch synthase | 13379977 | MtC90709_GC | 2 | 1 |
| 1 | Reversibly glycosylated polypeptide-1 | 7718398 | MtC10969_GC | 2 | 0 |
| 1 | 110-kD 4SnNc-Tudor | 7564626 | MtC50269.1_GC | 1 | 1 |
| 1 | Triosephosphate isomerase | 13380542 | MtC00059_GC | 1 | 0 |
| 1 | Thiamine biosynthesis protein | 7718895 | MtD00077_GC | 1 | 0 |
| 1 | Lipoxygenase | 6708795 | MtC60306_GC | 0 | 0 |
| 1 | S-adenosyl-l-homo-Cys hydrolase | 1710838 | MtC30011_GC | 0 | 0 |
| 2 | β-Tubulin | 7561503 | MtC00356.1_GC | 2 | 1 |
| 2 | Adenosine kinase | 10704292 | MtC10064_GC | 1 | 0 |
| 2 | Pyruvate DH β-subunit | 13780026 | MtC30080_GC | 0 | 0 |
| 2 | Myo-inositol-1-P synthase | 13369792 | MtC60071_GC | 0 | 0 |
| 3 | Heat shock cognate protein 80 | 14882556 | MtD00014_GC | 5 | 1 |
| 3 | Molecular chaperone BiP A | 14983890 | MtC00550.1_GC | 3 | 0 |
| 3 | Heat shock protein 90 | 10706885 | MtC00079_GC | 3 | 0 |
| 3 | Putative homology not assigned | 9678016 | MtC30333_GC | 0 | 0 |
| 3 | Resistance gene analog protein | 13779916 | MtD02701_GC | 0 | 0 |
| 4 | Elongation factor EF-2 | 10698429 | MtC00166.1_GC | 2 | 0 |