Figure 5.

The induction of mRNAs of early and late wound-inducible defense genes in wild-type and PGβS-AS transgenic tomato plants using RNA gel-blot analyses. Total leaf RNA was isolated from leaves, collected at the times indicated, and 20 μg from each time period was separated by electrophoresis, transferred to nylon membranes, and exposed to film as described in “Materials and Methods.” A, Membranes were probed for the induction of early expressed mRNAs using full-length cDNA clones for allene oxide synthase (AOS), lipoxygenase (LOX), Prosystemin (ProSYS), and PG catalytic subunit (PGcat). B, Membranes were probed with full-length cDNAs clones for proteinase Inh I, proteinase Inh II, and CPI. An 18S ribosomal RNA gene probe was used as a loading control.