Table II.

The effect of selection schemes on TF

TF is defined as the percentage of embryos producing events. One-third of cocultivated embryos from each experiment were transferred to one of three selection media containing different concentrations of butafenacil (nanomolar). All selection steps were kept in the dark. The decrease in TF from Experiments 1 to 4 may be due to inferior embryo quality at the beginning of the winter season. Significant differences are detected between selection schemes 1 and 2, 2 and 3, and 3 and 1 based on paired Student's t test at P = 0.05.

Selections	Experiment 1	Experiment 2	Experiment 3	Experiment 4	Average
250-500-750	33/153(21.6%)	11/45(24.4%)	1/51(2.0%)	2/67(3.0%)	47/316(14.9%)
500-750-750	35/153(22.9%)	12/45(26.7%)	2/51(3.9%)	3/67(4.4%)	52/316(16.5%)
750-750-750	38/155(24.5%)	15/45(33.3%)	4/51(7.8%)	4/67(5.9%)	61/318(19.2%)