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. 2003 Oct;133(2):773–782. doi: 10.1104/pp.103.023010

Figure 3.

Figure 3.

Overproduction and purification of recombinant VuFeSOD. A, SDS gel (10% [w/v] acrylamide) stained with Coomassie. Lanes 1 and 6, Prestained molecular mass markers in kilodaltons. Lane 2, Wild-type BL21 cells (60 μg of protein). Lane 3, Transformed cells 1 h after isopropylthio-β-galactoside (IPTG) induction (60 μg of protein). Lane 4, Recombinant VuFeSOD after affinity-chromatography purification (5 μg of protein). Lane 5, Same as lane 4 treated with thrombin (5 μg of protein). B, Native gel (15% [w/v] acrylamide) stained with Coomassie. Lane 1, Wild-type BL21 cells (60 μg of protein). Lane 2, Transformed cells after 1-h induction with IPTG (60 μg of protein). Lane 3, Purified recombinant VuFeSOD (5 μg of protein). Lane 4, Same as lane 3 treated with thrombin (5 μg of protein).