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. 2003 Oct;133(2):773–782. doi: 10.1104/pp.103.023010

Figure 5.

Figure 5.

Immunoblot analysis of recombinant VuFeSOD in legume nodule extracts. Samples were subjected to 10% (w/v) SDS-PAGE and proven with anti-VuFeSOD antibody. Lanes 1 and 10, Prestained molecular mass markers in kilodaltons. Lane 2, Purified recombinant VuFeSOD treated with thrombin. Lane 3, Cowpea nodules (60 μg). Lane 4, Cowpea leaves. Lane 5, Cowpea bacteroids. Lane 6, Soybean nodules. Lane 7, Common bean (Phaseolus vulgaris) nodules. Lane 8, Alfalfa nodules. Lane 9, Pea nodules. All lanes were loaded with 60 μg of protein, except lane 2, which was loaded with 0.2 μg of protein.