Figure 5.

Gel mobility shift assay using DNA fragments containing four tandem copies of EE-2 (o4xEE2) and its derivatives. A, ³²P-labeled double-stranded oligonucleotide of o4xEE2 (lane 1) and modified oligonucleotides carrying linker-scan mutations (lanes 2–4) were incubated with 2.5 μg of nuclear proteins purified from cells grown under low-CO₂ conditions in light. Probes o4xLS12 to o4xLS14 carry identical mutations to those in pLS12 to pLS14, respectively (Fig. 2). F indicates the free probe. B, Nucleotide sequences of o4xEE2 and modified oligonucleotides including mutations. Mutations that abolished C-III complex formation are highlighted.