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Comparison of the nucleotide sequences of EE-1 and EE-2, and cross-competition assays using oEE1 and o4xEE2 probes. A, Conserved nucleotides between EE-1 and EE-2 are boxed. In oLS1 and o4xLS13, nucleotide substitutions are shown by lowercase letters. B, 32P-labeled oEE1 (lanes 1–5) and 32P-labeled-o4xEE2 (lanes 6–10) probes were incubated with 600-fold molar excess amounts of unlabeled competitors as indicated above. F indicates the free probe.
