Table II.
Primers used in making linker-scan constructs
| Primera | Sequence (5′-3′)b | Position (5′-3′)c | Construct |
|---|---|---|---|
| LS1-f | CCGCCCGGTTGGAAGGAGGTTTGACGCGAG | -284 to -259 | pCT362LS1 |
| LS2-f | CCGCTTGGAAGGAGGTTTGACGCGAGGGAT | -280 to -255 | pCT362LS2 |
| LS3-f | AAACAAGGAGGTTTGACGCGAGGGATTTAG | -276 to -251 | pCT362LS3 |
| LS4-f | CCGCAGGTTTGACGCGAGGGATTTAGGTGC | -272 to -247 | pCT362LS4 |
| LS5-f | AAACTTGACGCGAGGGATTTAGGTGCGAGT | -268 to -243 | pCT362LS5 |
| LS6-f | AAACCGCGAGGGATTTAGGTGCGAGTCCGA | -264 to -239 | pCT362LS6 |
| LS7-f | CCGCAGGGATTTAGGTGCGAGTCCGACTTA | -260 to -235 | pCT362LS7 |
| LS8-f | CCGCATTTAGGTGCGAGTCCGACTTACGAA | -256 to -231 | pCT362LS8 |
| LS9-f | CCGCAGGTGCGAGTCCGACTTACGAAGGTA | -252 to -227 | pCT362LS9 |
| LS10-f | CCGCGCGAGTCCGACTTACGAAGGTACCAT | -248 to -223 | pCT362LS10 |
| LS11-f | AAACGACTTACGAAGGTACCATCGGGCCCC | -240 to -215 | pCT362LS11 |
| LS12-f | CCGCTACGAAGGTACCATCGGGCCCCCCCT | -236 to -211 | pCT362LS12 |
| LS13-f | CCGCAAGGTACCATCGGGCCCCCCCTCGAG | -232 to -207 | pCT362LS13 |
| LS14-f | CCGCTACCATCGGGCCCCCCCTCGAGGTCG | -228 to -203 | pCT362LS14 |
| LS1-r | CCGCTACAACGCTGCCAACGTGGTGGCGCG | -293 to -317 | pCT362LS1 |
| LS2-r | CCGCAATCTACAACGCTGCCAACGTGGTGG | -289 to -314 | pCT362LS2 |
| LS3-r | AAACTGAAAATCTACAACGCTGCCAACGTG | -285 to -310 | pCT362LS3 |
| LS4-r | CCGCCCGGTGAAAATCTACAACGCTGCCAA | -281 to -306 | pCT362LS4 |
| LS5-r | AAACCCAACCGGTGAAAATCTACAACGCTG | -277 to -302 | pCT362LS5 |
| LS6-r | AAACCCTTCCAACCGGTGAAAATCTACAAC | -273 to -298 | pCT362LS6 |
| LS7-r | CCGCACCTCCTTCCAACCGGTGAAAATCTA | -269 to -294 | pCT362LS7 |
| LS8-r | CCGCTCAAACCTCCTTCCAACCGGTGAAAA | -265 to -290 | pCT362LS8 |
| LS9-r | CCGCCGCGTCAAACCTCCTTCCAACCGGTG | -261 to -286 | pCT362LS9 |
| LS10-r | CCGCCCCTCGCGTCAAACCTCCTTCCAACC | -257 to -282 | pCT362LS10 |
| LS11-r | AAACACCTAAATCCCTCGCGTCAAACCTCC | -249 to -274 | pCT362LS11 |
| LS12-r | CCGCTCGCACCTAAATCCCTCGCGTCAAAC | -245 to -270 | pCT362LS12 |
| LS13-r | CCGCGGACTCGCACCTAAATCCCTCGCGTC | -241 to -266 | pCT362LS13 |
| LS14-r | CCGCAGTCGGACTCGCACCTAAATCCCTCG | -237 to -262 | pCT362LS14 |
a Forward primers (LSn-f) are designed along with Ars coding strand, and reverse primers (LSn-r) are designed along with complementary strand of Ars. b Underlined nucleotides indicate introduced mutations. c Mutated sequences in primers are not included in the numbers of positions.