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HPLC analyses for nitrilase activity toward IAN after 3 h of incubation. Reaction mixtures (100 μL) consisted of 50 mm potassium phosphate buffer (pH 8.0), 1 mm IAN, and bacterial crude extract (50 μg protein) containing no nitrilase (A), heterologously expressed ZmNIT1 (B), or ZmNIT2 (C).
