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. 2001 Apr 30;153(3):555–568. doi: 10.1083/jcb.153.3.555

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Northern analysis of cyclin D1 and c-myc mRNA levels in wt and ΔN89β-catenin mammary glands. mRNA was extracted from virgin (10 wk [10w Vir]), midpregnant (8 d [8d Preg]), and late pregnant (18 d [18d Preg]) mammary glands. Northern blots were prepared and probed with cDNAs encoding cyclin D1, c-myc, and K18 as a control to permit normalization for epithelial content of the mRNA samples. Note cyclin D1 and c-myc levels are abnormally elevated in transgenic (TG) virgin mice compared with their wt littermates.